RT Journal
A1 Kwitek, Anne E.
A1 Gullings-Handley, Jo
A1 Yu, Jiaming
A1 Carlos, Danilo C.
A1 Orlebeke, Kimberly
A1 Nie, Jeff
A1 Eckert, Jeffrey
A1 Lemke, Angela
A1 Andrae, Jaime Wendt
A1 Bromberg, Susan
A1 Pasko, Dean
A1 Chen, Dan
A1 Scheetz, Todd E.
A1 Casavant, Thomas L.
A1 Soares, M. Bento
A1 Sheffield, Val C.
A1 Tonellato, Peter J.
A1 Jacob, Howard J.
T1 High-Density Rat Radiation Hybrid Maps Containing Over 24,000 SSLPs, Genes, and ESTs Provide a Direct Link to the Rat Genome Sequence
JF Genome Research 
JO Genome Research 
YR 2004 
FD April 01 
VO 14 
IS 4 
SP 750 
OP 757 
DO 10.1101/gr.1968704 
UL http://genome.cshlp.org/content/14/4/750.abstract 
AB The laboratory rat is a major model organism for systems biology. To complement the cornucopia of physiological and pharmacological data generated in the rat, a large genomic toolset has been developed, culminating in the release of the rat draft genome sequence. The rat draft sequence used a variety of assembly packages, as well as data from the Radiation Hybrid (RH) map of the rat as part of their validation. As part of the Rat Genome Project, we have been building a high-density RH map to facilitate data integration from multiple maps and now to help validate the genome assembly. By incorporating vectors from our lab and several other labs, we have doubled the number of simple sequence length polymorphisms (SSLPs), genes, expressed sequence tags (ESTs), and sequence-tagged sites (STSs) compared to any other genome-wide rat map, a total of 24,437 elements. During the process, we also identified a novel approach for integrating the RH placement results from multiple maps. This new integrated RH map contains approximately 10 RH-mapped elements per Mb on the genome assembly, enabling the RH maps to serve as a scaffold for a variety of data visualization tools.