RT Journal
A1 Scheetz, Todd E.
A1 Laffin, Jennifer J.
A1 Berger, Brian
A1 Holte, Sara
A1 Baumes, Susan A.
A1 Brown, Robert
A1 Chang, Shereen
A1 Coco, Justin
A1 Conklin, Jim
A1 Crouch, Keith
A1 Donohue, Micca
A1 Doonan, Greg
A1 Estes, Chris
A1 Eyestone, Mari
A1 Fishler, Katrina
A1 Gardiner, Jack
A1 Guo, Lankai
A1 Johnson, Brad
A1 Keppel, Catherine
A1 Kreger, Rikki
A1 Lebeck, Mark
A1 Marcelino, Rudy
A1 Miljkovich, Vladan
A1 Perdue, Mindee
A1 Qui, Ling
A1 Rehmann, Joshua
A1 Reiter, Rebecca S.
A1 Rhoads, Bridgette
A1 Schaefer, Kelly
A1 Smith, Christina
A1 Sunjevaric, Ivana
A1 Trout, Kurtis
A1 Wu, Ning
A1 Birkett, Clayton L.
A1 Bischof, Jared
A1 Gackle, Barry
A1 Gavin, Allen
A1 Grundstad, A. Jason
A1 Mokrzycki, Brian
A1 Moressi, Chris
A1 O'Leary, Brian
A1 Pedretti, Kevin
A1 Roberts, Chad
A1 Robinson, Natalie L.
A1 Smith, Michael
A1 Tack, Dylan
A1 Trivedi, Nishank
A1 Kucaba, Tamara
A1 Freeman, Tom
A1 Lin, Jim J.-C.
A1 Bonaldo, Maria F.
A1 Casavant, Thomas L.
A1 Sheffield, Val C.
A1 Soares, M. Bento
T1 High-Throughput Gene Discovery in the Rat
JF Genome Research 
JO Genome Research 
YR 2004 
FD April 01 
VO 14 
IS 4 
SP 733 
OP 741 
DO 10.1101/gr.1414204 
UL http://genome.cshlp.org/content/14/4/733.abstract 
AB The rat is an important animal model for human diseases and is widely used in physiology. In this article we present a new strategy for gene discovery based on the production of ESTs from serially subtracted and normalized cDNA libraries, and we describe its application for the development of a comprehensive nonredundant collection of rat ESTs. Our new strategy appears to yield substantially more EST clusters per ESTs sequenced than do previous approaches that did not use serial subtraction. However, multiple rounds of library subtraction resulted in high frequencies of otherwise rare internally primed cDNAs, defining the limits of this powerful approach. To date, we have generated >200,000 3′ ESTs from >100 cDNA libraries representing a wide range of tissues and developmental stages of the laboratory rat. Most importantly, we have contributed to ∼50,000 rat UniGene clusters. We have identified, arrayed, and derived 5′ ESTs from >30,000 unique rat cDNA clones. Complete information, including radiation hybrid mapping data, is also maintained locally at http://genome.uiowa.edu/clcg.html. All of the sequences described in this article have been submitted to the dbEST division of the NCBI.