TY  - JOUR
A1  - Scheetz, Todd E.
A1  - Laffin, Jennifer J.
A1  - Berger, Brian
A1  - Holte, Sara
A1  - Baumes, Susan A.
A1  - Brown, Robert
A1  - Chang, Shereen
A1  - Coco, Justin
A1  - Conklin, Jim
A1  - Crouch, Keith
A1  - Donohue, Micca
A1  - Doonan, Greg
A1  - Estes, Chris
A1  - Eyestone, Mari
A1  - Fishler, Katrina
A1  - Gardiner, Jack
A1  - Guo, Lankai
A1  - Johnson, Brad
A1  - Keppel, Catherine
A1  - Kreger, Rikki
A1  - Lebeck, Mark
A1  - Marcelino, Rudy
A1  - Miljkovich, Vladan
A1  - Perdue, Mindee
A1  - Qui, Ling
A1  - Rehmann, Joshua
A1  - Reiter, Rebecca S.
A1  - Rhoads, Bridgette
A1  - Schaefer, Kelly
A1  - Smith, Christina
A1  - Sunjevaric, Ivana
A1  - Trout, Kurtis
A1  - Wu, Ning
A1  - Birkett, Clayton L.
A1  - Bischof, Jared
A1  - Gackle, Barry
A1  - Gavin, Allen
A1  - Grundstad, A. Jason
A1  - Mokrzycki, Brian
A1  - Moressi, Chris
A1  - O'Leary, Brian
A1  - Pedretti, Kevin
A1  - Roberts, Chad
A1  - Robinson, Natalie L.
A1  - Smith, Michael
A1  - Tack, Dylan
A1  - Trivedi, Nishank
A1  - Kucaba, Tamara
A1  - Freeman, Tom
A1  - Lin, Jim J.-C.
A1  - Bonaldo, Maria F.
A1  - Casavant, Thomas L.
A1  - Sheffield, Val C.
A1  - Soares, M. Bento
T1  - High-Throughput Gene Discovery in the Rat
Y1  - 2004/04/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 733 
EP  - 741 
DO  - 10.1101/gr.1414204 
VL  - 14 
IS  - 4 
UR  - http://genome.cshlp.org/content/14/4/733.abstract 
N2  - The rat is an important animal model for human diseases and is widely used in physiology. In this article we present a new strategy for gene discovery based on the production of ESTs from serially subtracted and normalized cDNA libraries, and we describe its application for the development of a comprehensive nonredundant collection of rat ESTs. Our new strategy appears to yield substantially more EST clusters per ESTs sequenced than do previous approaches that did not use serial subtraction. However, multiple rounds of library subtraction resulted in high frequencies of otherwise rare internally primed cDNAs, defining the limits of this powerful approach. To date, we have generated >200,000 3′ ESTs from >100 cDNA libraries representing a wide range of tissues and developmental stages of the laboratory rat. Most importantly, we have contributed to ∼50,000 rat UniGene clusters. We have identified, arrayed, and derived 5′ ESTs from >30,000 unique rat cDNA clones. Complete information, including radiation hybrid mapping data, is also maintained locally at http://genome.uiowa.edu/clcg.html. All of the sequences described in this article have been submitted to the dbEST division of the NCBI. 
ER  -