@article{Scheetz01042004,
author = {Scheetz, Todd E. and Laffin, Jennifer J. and Berger, Brian and Holte, Sara and Baumes, Susan A. and Brown, Robert and Chang, Shereen and Coco, Justin and Conklin, Jim and Crouch, Keith and Donohue, Micca and Doonan, Greg and Estes, Chris and Eyestone, Mari and Fishler, Katrina and Gardiner, Jack and Guo, Lankai and Johnson, Brad and Keppel, Catherine and Kreger, Rikki and Lebeck, Mark and Marcelino, Rudy and Miljkovich, Vladan and Perdue, Mindee and Qui, Ling and Rehmann, Joshua and Reiter, Rebecca S. and Rhoads, Bridgette and Schaefer, Kelly and Smith, Christina and Sunjevaric, Ivana and Trout, Kurtis and Wu, Ning and Birkett, Clayton L. and Bischof, Jared and Gackle, Barry and Gavin, Allen and Grundstad, A. Jason and Mokrzycki, Brian and Moressi, Chris and O'Leary, Brian and Pedretti, Kevin and Roberts, Chad and Robinson, Natalie L. and Smith, Michael and Tack, Dylan and Trivedi, Nishank and Kucaba, Tamara and Freeman, Tom and Lin, Jim J.-C. and Bonaldo, Maria F. and Casavant, Thomas L. and Sheffield, Val C. and Soares, M. Bento}, 
title = {High-Throughput Gene Discovery in the Rat},
volume = {14}, 
number = {4}, 
pages = {733-741}, 
year = {2004}, 
doi = {10.1101/gr.1414204}, 
abstract ={The rat is an important animal model for human diseases and is widely used in physiology. In this article we present a new strategy for gene discovery based on the production of ESTs from serially subtracted and normalized cDNA libraries, and we describe its application for the development of a comprehensive nonredundant collection of rat ESTs. Our new strategy appears to yield substantially more EST clusters per ESTs sequenced than do previous approaches that did not use serial subtraction. However, multiple rounds of library subtraction resulted in high frequencies of otherwise rare internally primed cDNAs, defining the limits of this powerful approach. To date, we have generated >200,000 3′ ESTs from >100 cDNA libraries representing a wide range of tissues and developmental stages of the laboratory rat. Most importantly, we have contributed to ∼50,000 rat UniGene clusters. We have identified, arrayed, and derived 5′ ESTs from >30,000 unique rat cDNA clones. Complete information, including radiation hybrid mapping data, is also maintained locally at http://genome.uiowa.edu/clcg.html. All of the sequences described in this article have been submitted to the dbEST division of the NCBI.}, 
URL = {http://genome.cshlp.org/content/14/4/733.abstract}, 
eprint = {http://genome.cshlp.org/content/14/4/733.full.pdf+html}, 
journal = {Genome Research} 
}