TY  - JOUR
A1  - Matsuzaki, Hajime
A1  - Loi, Halina
A1  - Dong, Shoulian
A1  - Tsai, Ya-Yu
A1  - Fang, Joy
A1  - Law, Jane
A1  - Di, Xiaojun
A1  - Liu, Wei-Min
A1  - Yang, Geoffrey
A1  - Liu, Guoying
A1  - Huang, Jing
A1  - Kennedy, Giulia C.
A1  - Ryder, Thomas B.
A1  - Marcus, Gregory A.
A1  - Walsh, P. Sean
A1  - Shriver, Mark D.
A1  - Puck, Jennifer M.
A1  - Jones, Keith W.
A1  - Mei, Rui
T1  - Parallel Genotyping of Over 10,000 SNPs Using a One-Primer Assay on a High-Density Oligonucleotide Array
Y1  - 2004/03/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 414 
EP  - 425 
DO  - 10.1101/gr.2014904 
VL  - 14 
IS  - 3 
UR  - http://genome.cshlp.org/content/14/3/414.abstract 
N2  - The analysis of single nucleotide polymorphisms (SNPs) is increasingly utilizedto investigate the genetic causes of complex human diseases. Here we present a high-throughput genotyping platform that uses a one-primer assay to genotype over 10,000 SNPs per individual on a single oligonucleotide array. This approach uses restriction digestion to fractionate the genome, followed by amplification of a specific fractionated subset of the genome. The resulting reduction in genome complexity enables allele-specific hybridization to the array. The selection of SNPs was primarily determined by computer-predicted lengths of restriction fragments containing the SNPs, andwas further driven by strict empirical measurements of accuracy, reproducibility, andaverage call rate, which we estimate to be >9.5%, >99.9%, and>95%, respectively. With average heterozygosity of 0.38 andgenome scan resolution of 0.31 cM, the SNP array is a viable alternative to panels of microsatellites (STRs). As a demonstration of the utility of the genotyping platform in whole-genome scans, we have replicated and refined a linkage region on chromosome 2p for chronic mucocutaneous candidiasis and thyroid disease, previously identified using a panel of microsatellite (STR) markers. 
ER  -