TY  - JOUR
A1  - Castelli, Vanina
A1  - Aury, Jean-Marc
A1  - Jaillon, Olivier
A1  - Wincker, Patrick
A1  - Clepet, Christian
A1  - Menard, Manuella
A1  - Cruaud, Corinne
A1  - Quétier, Francis
A1  - Scarpelli, Claude
A1  - Schächter, Vincent
A1  - Temple, Gary
A1  - Caboche, Michel
A1  - Weissenbach, Jean
A1  - Salanoubat, Marcel
T1  - Whole Genome Sequence Comparisons and “Full-Length” cDNA Sequences: A Combined Approach to Evaluate and Improve Arabidopsis Genome Annotation
Y1  - 2004/03/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 406 
EP  - 413 
DO  - 10.1101/gr.1515604 
VL  - 14 
IS  - 3 
UR  - http://genome.cshlp.org/content/14/3/406.abstract 
N2  - To evaluate the existing annotation of the Arabidopsis genome further, we generated a collection of evolutionary conserved regions (ecores) between Arabidopsis and rice. The ecore analysis provides evidence that the gene catalog of Arabidopsis is not yet complete, and that a number of these annotations require re-examination. To improve the Arabidopsis genome annotation further, we used a novel “full-length” enriched cDNA collection prepared from several tissues. An additional 1931 genes were covered by new “full-length” cDNA sequences, raising the number of annotated genes with a corresponding “full-length” cDNA sequence to about 14,000. Detailed comparisons between these “full-length” cDNA sequences and annotated genes show that this resource is very helpful in determining the correct structure of genes, in particular, those not yet supported by “full-length” cDNAs. In addition, a total of 326 genomic regions not included previously in the Arabidopsis genome annotation were detected by this cDNA resource, providing clues for new gene discovery. Because, as expected, the two data sets only partially overlap, their combination produces very useful information for improving the Arabidopsis genome annotation. 
ER  -