RT Journal
A1 Wu, Chengcang
A1 Sun, Shuku
A1 Nimmakayala, Padmavathi
A1 Santos, Felipe A.
A1 Meksem, Khalid
A1 Springman, Rachael
A1 Ding, Kejiao
A1 Lightfoot, David A.
A1 Zhang, Hong-Bin
T1 A BAC- and BIBAC-Based Physical Map of the Soybean Genome
JF Genome Research 
JO Genome Research 
YR 2004 
FD February 01 
VO 14 
IS 2 
SP 319 
OP 326 
DO 10.1101/gr.1405004 
UL http://genome.cshlp.org/content/14/2/319.abstract 
AB Genome-wide physical maps are crucial to many aspects of advanced genome research. We report a genome-wide, bacterial artificial chromosome (BAC) and plant-transformation-competent binary large-insert plasmid clone (hereafter BIBAC)-based physical map of the soybean genome. The map was constructed from 78,001 clones from five soybean BAC and BIBAC libraries representing 9.6 haploid genomes and three cultivars, and consisted of 2905 BAC/BIBAC contigs, estimated to span 1408 Mb in physical length. We evaluated the reliability of the map contigs using different contig assembly strategies, independent contig building methods, DNA marker hybridization, and different fingerprinting methods, and the results showed that the contigs were assembled properly. Furthermore, we tested the feasibility of integrating the physical map with the existing soybean composite genetic map using 388 DNA markers. The results further confirmed the nature of the ancient tetraploid origin of soybean and indicated that it is feasible to integrate the physical map with the linkage map even though greater efforts are needed. This map represents the first genome-wide, BAC/BIBAC-based physical map of the soybean genome and would provide a platform for advanced genome research of soybean and other legume species. The inclusion of BIBACs in the map would streamline the utility of the map for positional cloning of genes and QTLs, and functional analysis of soybean genomic sequences.