TY  - JOUR
A1  - Shigemori, Yasushi
A1  - Haruta, Hirotaka
A1  - Okada, Takao
A1  - Oishi, Michio
T1  - Marking of specific sequences in double-stranded DNA molecules—SNP detection and direct observation
Y1  - 2004/12/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 2478 
EP  - 2485 
DO  - 10.1101/gr.2789604 
VL  - 14 
IS  - 12 
UR  - http://genome.cshlp.org/content/14/12/2478.abstract 
N2  - In this study, we describe a simple method to mark specific sequences in double-stranded DNA molecules. For the marking, we used two specifically designed oligonucleotides, one of which is complementary to the sequence to be marked and the other, serving as a splint, to make the marking stable and detectable by subsequent various analytical means. In the presence of the two deoxyoligonucleotides, whereas RecA protein-mediated reaction converts the sequence to be marked to a regional triple-stranded structure with the complementary (probing) oligonucleotide, DNA ligase transforms it to a stable multi- (possibly quintuple) stranded structure with the splint oligonucleotide. The whole marking process is simple and completed in a single reaction mixture. Because RecA protein makes the marking to proceed with high fidelity, we were able to mark (detect) SNPs in complex genomes like human's. Furthermore, the structure of the marked sequence is stable and quite distinct enough to be readily detectable by biochemical means or direct observation by scanning probe microscopy. 
ER  -