TY  - JOUR
A1  - Abe, Kuniya
A1  - Noguchi, Hideki
A1  - Tagawa, Keiko
A1  - Yuzuriha, Misako
A1  - Toyoda, Atsushi
A1  - Kojima, Toshio
A1  - Ezawa, Kiyoshi
A1  - Saitou, Naruya
A1  - Hattori, Masahira
A1  - Sakaki, Yoshiyuki
A1  - Moriwaki, Kazuo
A1  - Shiroishi, Toshihiko
T1  - Contribution of Asian mouse subspecies Mus musculus molossinus to genomic constitution of strain C57BL/6J, as defined by BAC-end sequence–SNP analysis
Y1  - 2004/12/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 2439 
EP  - 2447 
DO  - 10.1101/gr.2899304 
VL  - 14 
IS  - 12 
UR  - http://genome.cshlp.org/content/14/12/2439.abstract 
N2  - MSM/Ms is an inbred strain derived from the Japanese wild mouse, Mus musculus molossinus. It is believed that subspecies molossinus has contributed substantially to the genome constitution of common laboratory strains of mice, although the majority of their genome is derived from the west European M. m. domesticus. Information on the molossinus genome is thus essential not only for genetic studies involving molossinus but also for characterization of common laboratory strains. Here, we report the construction of an arrayed bacterial artificial chromosome (BAC) library from male MSM/Ms genomic DNA, covering ∼1× genome equivalent. Both ends of 176,256 BAC clone inserts were sequenced, and 62,988 BAC-end sequence (BES) pairs were mapped onto the C57BL/6J genome (NCBI mouse Build 30), covering 2,228,164 kbp or 89% of the total genome. Taking advantage of the BES map data, we established a computer-based clone screening system. Comparison of the MSM/Ms and C57BL/6J sequences revealed 489,200 candidate single nucleotide polymorphisms (SNPs) in 51,137,941 bp sequenced. The overall nucleotide substitution rate was as high as 0.0096. The distribution of SNPs along the C57BL/6J genome was not uniform: The majority of the genome showed a high SNP rate, and only 5.2% of the genome showed an extremely low SNP rate (percentage identity = 0.9997); these sequences are likely derived from the molossinus genome. 
ER  -