RT Journal
A1 Dricot, Amélie
A1 Rual, Jean-François
A1 Lamesch, Philippe
A1 Bertin, Nicolas
A1 Dupuy, Denis
A1 Hao, Tong
A1 Lambert, Christophe
A1 Hallez, Régis
A1 Delroisse, Jean-Marc
A1 Vandenhaute, Jean
A1 Lopez-Goñi, Ignacio
A1 Moriyon, Ignacio
A1 Garcia-Lobo, Juan M.
A1 Sangari, Félix J.
A1 MacMillan, Alastair P.
A1 Cutler, Sally J.
A1 Whatmore, Adrian M.
A1 Bozak, Stephanie
A1 Sequerra, Reynaldo
A1 Doucette-Stamm, Lynn
A1 Vidal, Marc
A1 Hill, David E.
A1 Letesson, Jean-Jacques
A1 De Bolle, Xavier
T1 Generation of the Brucella melitensis ORFeome Version 1.1
JF Genome Research 
JO Genome Research 
YR 2004 
FD October 15 
VO 14 
IS 10b 
SP 2201 
OP 2206 
DO 10.1101/gr.2456204 
UL http://genome.cshlp.org/content/14/10b/2201.abstract 
AB The bacteria of the Brucella genus are responsible for a worldwide zoonosis called brucellosis. They belong to the α-proteobacteria group, as many other bacteria that live in close association with a eukaryotic host. Importantly, the Brucellae are mainly intracellular pathogens, and the molecular mechanisms of their virulence are still poorly understood. Using the complete genome sequence of Brucella melitensis, we generated a database of protein-coding open reading frames (ORFs) and constructed an ORFeome library of 3091 Gateway Entry clones, each containing a defined ORF. This first version of the Brucella ORFeome (v1.1) provides the coding sequences in a user-friendly format amenable to high-throughput functional genomic and proteomic experiments, as the ORFs are conveniently transferable from the Entry clones to various Expression vectors by recombinational cloning. The cloning of the Brucella ORFeome v1.1 should help to provide a better understanding of the molecular mechanisms of virulence, including the identification of bacterial protein-protein interactions, but also interactions between bacterial effectors and their host's targets.