TY  - JOUR
A1  - Dricot, Amélie
A1  - Rual, Jean-François
A1  - Lamesch, Philippe
A1  - Bertin, Nicolas
A1  - Dupuy, Denis
A1  - Hao, Tong
A1  - Lambert, Christophe
A1  - Hallez, Régis
A1  - Delroisse, Jean-Marc
A1  - Vandenhaute, Jean
A1  - Lopez-Goñi, Ignacio
A1  - Moriyon, Ignacio
A1  - Garcia-Lobo, Juan M.
A1  - Sangari, Félix J.
A1  - MacMillan, Alastair P.
A1  - Cutler, Sally J.
A1  - Whatmore, Adrian M.
A1  - Bozak, Stephanie
A1  - Sequerra, Reynaldo
A1  - Doucette-Stamm, Lynn
A1  - Vidal, Marc
A1  - Hill, David E.
A1  - Letesson, Jean-Jacques
A1  - De Bolle, Xavier
T1  - Generation of the Brucella melitensis ORFeome Version 1.1
Y1  - 2004/10/15 
JF  - Genome Research 
JO  - Genome Research 
SP  - 2201 
EP  - 2206 
DO  - 10.1101/gr.2456204 
VL  - 14 
IS  - 10b 
UR  - http://genome.cshlp.org/content/14/10b/2201.abstract 
N2  - The bacteria of the Brucella genus are responsible for a worldwide zoonosis called brucellosis. They belong to the α-proteobacteria group, as many other bacteria that live in close association with a eukaryotic host. Importantly, the Brucellae are mainly intracellular pathogens, and the molecular mechanisms of their virulence are still poorly understood. Using the complete genome sequence of Brucella melitensis, we generated a database of protein-coding open reading frames (ORFs) and constructed an ORFeome library of 3091 Gateway Entry clones, each containing a defined ORF. This first version of the Brucella ORFeome (v1.1) provides the coding sequences in a user-friendly format amenable to high-throughput functional genomic and proteomic experiments, as the ORFs are conveniently transferable from the Entry clones to various Expression vectors by recombinational cloning. The cloning of the Brucella ORFeome v1.1 should help to provide a better understanding of the molecular mechanisms of virulence, including the identification of bacterial protein-protein interactions, but also interactions between bacterial effectors and their host's targets. 
ER  -