RT Journal
A1 Hilson, Pierre
A1 Allemeersch, Joke
A1 Altmann, Thomas
A1 Aubourg, Sébastien
A1 Avon, Alexandra
A1 Beynon, Jim
A1 Bhalerao, Rishikesh P.
A1 Bitton, Frédérique
A1 Caboche, Michel
A1 Cannoot, Bernard
A1 Chardakov, Vasil
A1 Cognet-Holliger, Cécile
A1 Colot, Vincent
A1 Crowe, Mark
A1 Darimont, Caroline
A1 Durinck, Steffen
A1 Eickhoff, Holger
A1 de Longevialle, Andéol Falcon
A1 Farmer, Edward E.
A1 Grant, Murray
A1 Kuiper, Martin T.R.
A1 Lehrach, Hans
A1 Léon, Céline
A1 Leyva, Antonio
A1 Lundeberg, Joakim
A1 Lurin, Claire
A1 Moreau, Yves
A1 Nietfeld, Wilfried
A1 Paz-Ares, Javier
A1 Reymond, Philippe
A1 Rouzé, Pierre
A1 Sandberg, Goran
A1 Segura, Maria Dolores
A1 Serizet, Carine
A1 Tabrett, Alexandra
A1 Taconnat, Ludivine
A1 Thareau, Vincent
A1 Van Hummelen, Paul
A1 Vercruysse, Steven
A1 Vuylsteke, Marnik
A1 Weingartner, Magdalena
A1 Weisbeek, Peter J.
A1 Wirta, Valtteri
A1 Wittink, Floyd R.A.
A1 Zabeau, Marc
A1 Small, Ian
T1 Versatile Gene-Specific Sequence Tags for Arabidopsis Functional Genomics: Transcript Profiling and Reverse Genetics Applications
JF Genome Research 
JO Genome Research 
YR 2004 
FD October 15 
VO 14 
IS 10b 
SP 2176 
OP 2189 
DO 10.1101/gr.2544504 
UL http://genome.cshlp.org/content/14/10b/2176.abstract 
AB Microarray transcript profiling and RNA interference are two new technologies crucial for large-scale gene function studies in multicellular eukaryotes. Both rely on sequence-specific hybridization between complementary nucleic acid strands, inciting us to create a collection of gene-specific sequence tags (GSTs) representing at least 21,500 Arabidopsis genes and which are compatible with both approaches. The GSTs were carefully selected to ensure that each of them shared no significant similarity with any other region in the Arabidopsis genome. They were synthesized by PCR amplification from genomic DNA. Spotted microarrays fabricated from the GSTs show good dynamic range, specificity, and sensitivity in transcript profiling experiments. The GSTs have also been transferred to bacterial plasmid vectors via recombinational cloning protocols. These cloned GSTs constitute the ideal starting point for a variety of functional approaches, including reverse genetics. We have subcloned GSTs on a large scale into vectors designed for gene silencing in plant cells. We show that in planta expression of GST hairpin RNA results in the expected phenotypes in silenced Arabidopsis lines. These versatile GST resources provide novel and powerful tools for functional genomics.