TY  - JOUR
A1  - Hilson, Pierre
A1  - Allemeersch, Joke
A1  - Altmann, Thomas
A1  - Aubourg, Sébastien
A1  - Avon, Alexandra
A1  - Beynon, Jim
A1  - Bhalerao, Rishikesh P.
A1  - Bitton, Frédérique
A1  - Caboche, Michel
A1  - Cannoot, Bernard
A1  - Chardakov, Vasil
A1  - Cognet-Holliger, Cécile
A1  - Colot, Vincent
A1  - Crowe, Mark
A1  - Darimont, Caroline
A1  - Durinck, Steffen
A1  - Eickhoff, Holger
A1  - de Longevialle, Andéol Falcon
A1  - Farmer, Edward E.
A1  - Grant, Murray
A1  - Kuiper, Martin T.R.
A1  - Lehrach, Hans
A1  - Léon, Céline
A1  - Leyva, Antonio
A1  - Lundeberg, Joakim
A1  - Lurin, Claire
A1  - Moreau, Yves
A1  - Nietfeld, Wilfried
A1  - Paz-Ares, Javier
A1  - Reymond, Philippe
A1  - Rouzé, Pierre
A1  - Sandberg, Goran
A1  - Segura, Maria Dolores
A1  - Serizet, Carine
A1  - Tabrett, Alexandra
A1  - Taconnat, Ludivine
A1  - Thareau, Vincent
A1  - Van Hummelen, Paul
A1  - Vercruysse, Steven
A1  - Vuylsteke, Marnik
A1  - Weingartner, Magdalena
A1  - Weisbeek, Peter J.
A1  - Wirta, Valtteri
A1  - Wittink, Floyd R.A.
A1  - Zabeau, Marc
A1  - Small, Ian
T1  - Versatile Gene-Specific Sequence Tags for Arabidopsis Functional Genomics: Transcript Profiling and Reverse Genetics Applications
Y1  - 2004/10/15 
JF  - Genome Research 
JO  - Genome Research 
SP  - 2176 
EP  - 2189 
DO  - 10.1101/gr.2544504 
VL  - 14 
IS  - 10b 
UR  - http://genome.cshlp.org/content/14/10b/2176.abstract 
N2  - Microarray transcript profiling and RNA interference are two new technologies crucial for large-scale gene function studies in multicellular eukaryotes. Both rely on sequence-specific hybridization between complementary nucleic acid strands, inciting us to create a collection of gene-specific sequence tags (GSTs) representing at least 21,500 Arabidopsis genes and which are compatible with both approaches. The GSTs were carefully selected to ensure that each of them shared no significant similarity with any other region in the Arabidopsis genome. They were synthesized by PCR amplification from genomic DNA. Spotted microarrays fabricated from the GSTs show good dynamic range, specificity, and sensitivity in transcript profiling experiments. The GSTs have also been transferred to bacterial plasmid vectors via recombinational cloning protocols. These cloned GSTs constitute the ideal starting point for a variety of functional approaches, including reverse genetics. We have subcloned GSTs on a large scale into vectors designed for gene silencing in plant cells. We show that in planta expression of GST hairpin RNA results in the expected phenotypes in silenced Arabidopsis lines. These versatile GST resources provide novel and powerful tools for functional genomics. 
ER  -