RT Journal
A1 Wiemann, Stefan
A1 Arlt, Dorit
A1 Huber, Wolfgang
A1 Wellenreuther, Ruth
A1 Schleeger, Simone
A1 Mehrle, Alexander
A1 Bechtel, Stephanie
A1 Sauermann, Mamatha
A1 Korf, Ulrike
A1 Pepperkok, Rainer
A1 Sültmann, Holger
A1 Poustka, Annemarie
T1 From ORFeome to Biology: A Functional Genomics Pipeline
JF Genome Research 
JO Genome Research 
YR 2004 
FD October 15 
VO 14 
IS 10b 
SP 2136 
OP 2144 
DO 10.1101/gr.2576704 
UL http://genome.cshlp.org/content/14/10b/2136.abstract 
AB As several model genomes have been sequenced, the elucidation of protein function is the next challenge toward the understanding of biological processes in health and disease. We have generated a human ORFeome resource and established a functional genomics and proteomics analysis pipeline to address the major topics in the post-genome-sequencing era: the identification of human genes and splice forms, and the determination of protein localization, activity, and interaction. Combined with the understanding of when and where gene products are expressed in normal and diseased conditions, we create information that is essential for understanding the interplay of genes and proteins in the complex biological network. We have implemented bioinformatics tools and databases that are suitable to store, analyze, and integrate the different types of data from high-throughput experiments and to include further annotation that is based on external information. All information is presented in a Web database (http://www.dkfz.de/LIFEdb). It is exploited for the identification of disease-relevant genes and proteins for diagnosis and therapy.