RT Journal
A1 Rual, Jean-François
A1 Hirozane-Kishikawa, Tomoko
A1 Hao, Tong
A1 Bertin, Nicolas
A1 Li, Siming
A1 Dricot, Amélie
A1 Li, Ning
A1 Rosenberg, Jennifer
A1 Lamesch, Philippe
A1 Vidalain, Pierre-Olivier
A1 Clingingsmith, Tracey R.
A1 Hartley, James L.
A1 Esposito, Dominic
A1 Cheo, David
A1 Moore, Troy
A1 Simmons, Blake
A1 Sequerra, Reynaldo
A1 Bosak, Stephanie
A1 Doucette-Stamm, Lynn
A1 Le Peuch, Christian
A1 Vandenhaute, Jean
A1 Cusick, Michael E.
A1 Albala, Joanna S.
A1 Hill, David E.
A1 Vidal, Marc
T1 Human ORFeome Version 1.1: A Platform for Reverse Proteomics
JF Genome Research 
JO Genome Research 
YR 2004 
FD October 15 
VO 14 
IS 10b 
SP 2128 
OP 2135 
DO 10.1101/gr.2973604 
UL http://genome.cshlp.org/content/14/10b/2128.abstract 
AB The advent of systems biology necessitates the cloning of nearly entire sets of protein-encoding open reading frames (ORFs), or ORFeomes, to allow functional studies of the corresponding proteomes. Here, we describe the generation of a first version of the human ORFeome using a newly improved Gateway recombinational cloning approach. Using the Mammalian Gene Collection (MGC) resource as a starting point, we report the successful cloning of 8076 human ORFs, representing at least 7263 human genes, as mini-pools of PCR-amplified products. These were assembled into the human ORFeome version 1.1 (hORFeome v1.1) collection. After assessing the overall quality of this version, we describe the use of hORFeome v1.1 for heterologous protein expression in two different expression systems at proteome scale. The hORFeome v1.1 represents a central resource for the cloning of large sets of human ORFs in various settings for functional proteomics of many types, and will serve as the foundation for subsequent improved versions of the human ORFeome.