TY  - JOUR
A1  - Rual, Jean-François
A1  - Hirozane-Kishikawa, Tomoko
A1  - Hao, Tong
A1  - Bertin, Nicolas
A1  - Li, Siming
A1  - Dricot, Amélie
A1  - Li, Ning
A1  - Rosenberg, Jennifer
A1  - Lamesch, Philippe
A1  - Vidalain, Pierre-Olivier
A1  - Clingingsmith, Tracey R.
A1  - Hartley, James L.
A1  - Esposito, Dominic
A1  - Cheo, David
A1  - Moore, Troy
A1  - Simmons, Blake
A1  - Sequerra, Reynaldo
A1  - Bosak, Stephanie
A1  - Doucette-Stamm, Lynn
A1  - Le Peuch, Christian
A1  - Vandenhaute, Jean
A1  - Cusick, Michael E.
A1  - Albala, Joanna S.
A1  - Hill, David E.
A1  - Vidal, Marc
T1  - Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Y1  - 2004/10/15 
JF  - Genome Research 
JO  - Genome Research 
SP  - 2128 
EP  - 2135 
DO  - 10.1101/gr.2973604 
VL  - 14 
IS  - 10b 
UR  - http://genome.cshlp.org/content/14/10b/2128.abstract 
N2  - The advent of systems biology necessitates the cloning of nearly entire sets of protein-encoding open reading frames (ORFs), or ORFeomes, to allow functional studies of the corresponding proteomes. Here, we describe the generation of a first version of the human ORFeome using a newly improved Gateway recombinational cloning approach. Using the Mammalian Gene Collection (MGC) resource as a starting point, we report the successful cloning of 8076 human ORFs, representing at least 7263 human genes, as mini-pools of PCR-amplified products. These were assembled into the human ORFeome version 1.1 (hORFeome v1.1) collection. After assessing the overall quality of this version, we describe the use of hORFeome v1.1 for heterologous protein expression in two different expression systems at proteome scale. The hORFeome v1.1 represents a central resource for the cloning of large sets of human ORFs in various settings for functional proteomics of many types, and will serve as the foundation for subsequent improved versions of the human ORFeome. 
ER  -