RT Journal
A1 Bonaldo, Maria F.
A1 Bair, Thomas B.
A1 Scheetz, Todd E.
A1 Snir, Einat
A1 Akabogu, Ike
A1 Bair, Jennifer L.
A1 Berger, Brian
A1 Crouch, Keith
A1 Davis, Aja
A1 Eyestone, Mari E.
A1 Keppel, Catherine
A1 Kucaba, Tamara A.
A1 Lebeck, Mark
A1 Lin, Jenny L.
A1 de Melo, Anna I.R.
A1 Rehmann, Joshua
A1 Reiter, Rebecca S.
A1 Schaefer, Kelly
A1 Smith, Christina
A1 Tack, Dylan
A1 Trout, Kurtis
A1 Sheffield, Val C.
A1 Lin, Jim J-C.
A1 Casavant, Thomas L.
A1 Soares, Marcelo B.
T1 1274 Full-Open Reading Frames of Transcripts Expressed in the Developing Mouse Nervous System
JF Genome Research 
JO Genome Research 
YR 2004 
FD October 15 
VO 14 
IS 10b 
SP 2053 
OP 2063 
DO 10.1101/gr.2601304 
UL http://genome.cshlp.org/content/14/10b/2053.abstract 
AB As part of the trans-National Institutes of Health (NIH) Mouse Brain Molecular Anatomy Project (BMAP), and in close coordination with the NIH Mammalian Gene Collection Program (MGC), we initiated a large-scale project to clone, identify, and sequence the complete open reading frame (ORF) of transcripts expressed in the developing mouse nervous system. Here we report the analysis of the ORF sequence of 1274 cDNAs, obtained from 47 full-length-enriched cDNA libraries, constructed by using a novel approach, herein described. cDNA libraries were derived from size-fractionated cytoplasmic mRNA isolated from brain and eye tissues obtained at several embryonic stages and postnatal days. Altogether, including the full-ORF MGC sequences derived from these libraries by the MGC sequencing team, NIH_BMAP full-ORF sequences correspond to ∼20% of all transcripts currently represented in mouse MGC. We show that NIH_BMAP clones comprise 68% of mouse MGC cDNAs ≥5 kb, and 54% of those ≥4 kb, as of March 15, 2004. Importantly, we identified transcripts, among the 1274 full-ORF sequences, that are exclusively or predominantly expressed in brain and eye tissues, many of which encode yet uncharacterized proteins.