TY  - JOUR
A1  - Bonaldo, Maria F.
A1  - Bair, Thomas B.
A1  - Scheetz, Todd E.
A1  - Snir, Einat
A1  - Akabogu, Ike
A1  - Bair, Jennifer L.
A1  - Berger, Brian
A1  - Crouch, Keith
A1  - Davis, Aja
A1  - Eyestone, Mari E.
A1  - Keppel, Catherine
A1  - Kucaba, Tamara A.
A1  - Lebeck, Mark
A1  - Lin, Jenny L.
A1  - de Melo, Anna I.R.
A1  - Rehmann, Joshua
A1  - Reiter, Rebecca S.
A1  - Schaefer, Kelly
A1  - Smith, Christina
A1  - Tack, Dylan
A1  - Trout, Kurtis
A1  - Sheffield, Val C.
A1  - Lin, Jim J-C.
A1  - Casavant, Thomas L.
A1  - Soares, Marcelo B.
T1  - 1274 Full-Open Reading Frames of Transcripts Expressed in the Developing Mouse Nervous System
Y1  - 2004/10/15 
JF  - Genome Research 
JO  - Genome Research 
SP  - 2053 
EP  - 2063 
DO  - 10.1101/gr.2601304 
VL  - 14 
IS  - 10b 
UR  - http://genome.cshlp.org/content/14/10b/2053.abstract 
N2  - As part of the trans-National Institutes of Health (NIH) Mouse Brain Molecular Anatomy Project (BMAP), and in close coordination with the NIH Mammalian Gene Collection Program (MGC), we initiated a large-scale project to clone, identify, and sequence the complete open reading frame (ORF) of transcripts expressed in the developing mouse nervous system. Here we report the analysis of the ORF sequence of 1274 cDNAs, obtained from 47 full-length-enriched cDNA libraries, constructed by using a novel approach, herein described. cDNA libraries were derived from size-fractionated cytoplasmic mRNA isolated from brain and eye tissues obtained at several embryonic stages and postnatal days. Altogether, including the full-ORF MGC sequences derived from these libraries by the MGC sequencing team, NIH_BMAP full-ORF sequences correspond to ∼20% of all transcripts currently represented in mouse MGC. We show that NIH_BMAP clones comprise 68% of mouse MGC cDNAs ≥5 kb, and 54% of those ≥4 kb, as of March 15, 2004. Importantly, we identified transcripts, among the 1274 full-ORF sequences, that are exclusively or predominantly expressed in brain and eye tissues, many of which encode yet uncharacterized proteins. 
ER  -