TY  - JOUR
A1  - Oyama, Masaaki
A1  - Itagaki, Chiharu
A1  - Hata, Hiroko
A1  - Suzuki, Yutaka
A1  - Izumi, Tomonori
A1  - Natsume, Tohru
A1  - Isobe, Toshiaki
A1  - Sugano, Sumio
T1  - Analysis of Small Human Proteins Reveals the Translation of Upstream Open Reading Frames of mRNAs
Y1  - 2004/10/15 
JF  - Genome Research 
JO  - Genome Research 
SP  - 2048 
EP  - 2052 
DO  - 10.1101/gr.2384604 
VL  - 14 
IS  - 10b 
UR  - http://genome.cshlp.org/content/14/10b/2048.abstract 
N2  - To find novel short coding sequences from accumulated full-length cDNA sequences, proteomic analysis of small proteins expressed in human leukemia K562 cells was performed using high-resolution nanoflow liquid chromatography coupled with electrospray ionization tandem mass spectrometry. Our analysis led to the identification of 54 proteins not more than 100 amino acids in length, including four novel ones. These novel short coding sequences were all located upstream of the longest open reading frame (ORF) of the corresponding cDNA. Our findings indicate that the translation of short ORFs occurs in vivo whether or not there exists a longer coding region in the downstream of the mRNA. This investigation provides the first direct evidence of translation of upstream ORFs in human cells, which could greatly change the current outline of the human proteome. 
ER  -