RT Journal
A1 Brasch, Michael A.
A1 Hartley, James L.
A1 Vidal, Marc
T1 ORFeome Cloning and Systems Biology: Standardized Mass Production of the Parts From the Parts-List
JF Genome Research 
JO Genome Research 
YR 2004 
FD October 15 
VO 14 
IS 10b 
SP 2001 
OP 2009 
DO 10.1101/gr.2769804 
UL http://genome.cshlp.org/content/14/10b/2001.abstract 
AB Together with metabolites, proteins and RNAs form complex biological systems through highly intricate networks of physical and functional interactions. Large-scale studies aimed at a molecular understanding of the structure, function, and dynamics of proteins and RNAs in the context of cellular networks require novel approaches and technologies. This Special Issue of Genome Research features strategies for the high-throughput construction and manipulation of complete sets of protein-encoding open reading frames (ORFeome), gene promoters (promoterome), and noncoding RNAs, as predicted from genome and transcriptome sequences. Here we discuss the use of a recombinational cloning system that allows efficiency, adaptability, and compatibility in the generation of ORFeome, promoterome, and other resources.