TY  - JOUR
A1  - Chung, Yeun-Jun
A1  - Jonkers, Jos
A1  - Kitson, Hannah
A1  - Fiegler, Heike
A1  - Humphray, Sean
A1  - Scott, Carol
A1  - Hunt, Sarah
A1  - Yu, Yuejin
A1  - Nishijima, Ichiko
A1  - Velds, Arno
A1  - Holstege, Henne
A1  - Carter, Nigel
A1  - Bradley, Allan
T1  - A Whole-Genome Mouse BAC Microarray With 1-Mb Resolution for Analysis of DNA Copy Number Changes by Array Comparative Genomic Hybridization
Y1  - 2004/01/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 188 
EP  - 196 
DO  - 10.1101/gr.1878804 
VL  - 14 
IS  - 1 
UR  - http://genome.cshlp.org/content/14/1/188.abstract 
N2  - Microarray-basedcomparative genomic hybridization (CGH) has become a powerful methodfor the genome-wide detection of chromosomal imbalances. Although BAC microarrays have been usedfor mouse CGH studies, the resolving power of these analyses was limitedbecause high-density whole-genome mouse BAC microarrays were not available. We therefore developed a mouse BAC microarray containing 2803 unique BAC clones from mouse genomic libraries at 1-Mb intervals. For the general amplification of BAC clone DNA prior to spotting, we designed a set of three novel degenerate oligonucleotide-primed (DOP) PCR primers that preferentially amplify mouse genomic sequences while minimizing unwantedamplification of contaminating Escherichia coli DNA. The resulting 3K mouse BAC microarrays reproducibly identified DNA copy number alterations in cell lines andprimary tumors, such as single-copy deletions, regional amplifications, and aneuploidy. 
ER  -