RT Journal
A1 Zhou, Shiguo
A1 Kvikstad, Erika
A1 Kile, Andrew
A1 Severin, Jessica
A1 Forrest, Dan
A1 Runnheim, Rod
A1 Churas, Chris
A1 Hickman, Jason W.
A1 Mackenzie, Chris
A1 Choudhary, Madhusudan
A1 Donohue, Timothy
A1 Kaplan, Samuel
A1 Schwartz, David C.
T1 Whole-Genome Shotgun Optical Mapping of Rhodobacter sphaeroides  strain 2.4.1 and Its Use for Whole-Genome Shotgun Sequence Assembly
JF Genome Research 
JO Genome Research 
YR 2003 
FD September 01 
VO 13 
IS 9 
SP 2142 
OP 2151 
DO 10.1101/gr.1128803 
UL http://genome.cshlp.org/content/13/9/2142.abstract 
AB Rhodobacter sphaeroides 2.4.1 is a facultative photoheterotrophic  bacterium with tremendous metabolic diversity, which has significantly  contributed to our understanding of the molecular genetics of photosynthesis,  photoheterotrophy, nitrogen fixation, hydrogen metabolism, carbon dioxide  fixation, taxis, and tetrapyrrole biosynthesis. To further understand this  remarkable bacterium, and to accelerate an ongoing sequencing project, two  whole-genome restriction maps (EcoRI and HindIII) of R.  sphaeroides strain 2.4.1 were constructed using shotgun optical mapping.  The approach directly mapped genomic DNA by the random mapping of single  molecules. The two maps were used to facilitate sequence assembly by providing  an optical scaffold for high-resolution alignment and verification of sequence  contigs. Our results show that such maps facilitated the closure of sequence  gaps by the early detection of nascent sequence contigs during the course of  the whole-genome shotgun sequencing process.