TY  - JOUR
A1  - Zhou, Shiguo
A1  - Kvikstad, Erika
A1  - Kile, Andrew
A1  - Severin, Jessica
A1  - Forrest, Dan
A1  - Runnheim, Rod
A1  - Churas, Chris
A1  - Hickman, Jason W.
A1  - Mackenzie, Chris
A1  - Choudhary, Madhusudan
A1  - Donohue, Timothy
A1  - Kaplan, Samuel
A1  - Schwartz, David C.
T1  - Whole-Genome Shotgun Optical Mapping of Rhodobacter sphaeroides  strain 2.4.1 and Its Use for Whole-Genome Shotgun Sequence Assembly
Y1  - 2003/09/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 2142 
EP  - 2151 
DO  - 10.1101/gr.1128803 
VL  - 13 
IS  - 9 
UR  - http://genome.cshlp.org/content/13/9/2142.abstract 
N2  - Rhodobacter sphaeroides 2.4.1 is a facultative photoheterotrophic  bacterium with tremendous metabolic diversity, which has significantly  contributed to our understanding of the molecular genetics of photosynthesis,  photoheterotrophy, nitrogen fixation, hydrogen metabolism, carbon dioxide  fixation, taxis, and tetrapyrrole biosynthesis. To further understand this  remarkable bacterium, and to accelerate an ongoing sequencing project, two  whole-genome restriction maps (EcoRI and HindIII) of R.  sphaeroides strain 2.4.1 were constructed using shotgun optical mapping.  The approach directly mapped genomic DNA by the random mapping of single  molecules. The two maps were used to facilitate sequence assembly by providing  an optical scaffold for high-resolution alignment and verification of sequence  contigs. Our results show that such maps facilitated the closure of sequence  gaps by the early detection of nascent sequence contigs during the course of  the whole-genome shotgun sequencing process. 
ER  -