RT Journal
A1 Versteeg,, Rogier
A1 van Schaik, Barbera D.C.
A1 van Batenburg, Marinus F.
A1 Roos, Marco
A1 Monajemi, Ramin
A1 Caron, Huib
A1 Bussemaker, Harmen J.
A1 van Kampen, Antoine H.C.
T1 The Human Transcriptome Map Reveals Extremes in Gene Density, Intron Length, GC Content, and Repeat Pattern for Domains of Highly and Weakly Expressed Genes
JF Genome Research 
JO Genome Research 
YR 2003 
FD September 01 
VO 13 
IS 9 
SP 1998 
OP 2004 
DO 10.1101/gr.1649303 
UL http://genome.cshlp.org/content/13/9/1998.abstract 
AB The chromosomal gene expression profiles established by the Human  Transcriptome Map (HTM) revealed a clustering of highly expressed genes in  about 30 domains, called ridges. To physically characterize ridges, we  constructed a new HTM based on the draft human genome sequence (HTMseq).  Expression of 25,003 genes can be analyzed online in a multitude of tissues  (http://bioinfo.amc.uva.nl/HTMseq).  Ridges are found to be very gene-dense domains with a high GC content, a high  SINE repeat density, and a low LINE repeat density. Genes in ridges have  significantly shorter introns than genes outside of ridges. The HTMseq also  identifies a significant clustering of weakly expressed genes in domains with  fully opposite characteristics (antiridges). Both types of domains are open to  tissue-specific expression regulation, but the maximal expression levels in  ridges are considerably higher than in antiridges. Ridges are therefore an  integral part of a higher order structure in the genome related to  transcriptional regulation.