TY  - JOUR
A1  - Larkin, Denis M.
A1  - Everts-van der Wind, Annelie
A1  - Rebeiz, Mark
A1  - Schweitzer, Peter A.
A1  - Bachman, Sharon
A1  - Green, Cheryl
A1  - Wright, Chris L.
A1  - Campos, Edhilvia J.
A1  - Benson, Leslie D.
A1  - Edwards, Jennifer
A1  - Liu, Lei
A1  - Osoegawa, Kazutoyo
A1  - Womack, James E.
A1  - de Jong, Pieter J.
A1  - Lewin, Harris A.
T1  - A Cattle–Human Comparative Map Built with Cattle BAC-Ends and Human Genome Sequence
Y1  - 2003/08/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1966 
EP  - 1972 
DO  - 10.1101/gr.1560203 
VL  - 13 
IS  - 8 
UR  - http://genome.cshlp.org/content/13/8/1966.abstract 
N2  - As a step toward the goal of adding the cattle genome to those available  for multispecies comparative genome analysis, 40,224 cattle BAC clones were  end-sequenced, yielding 60,547 sequences (BAC end sequences, BESs) after  trimming with an average read length of 515 bp. Cattle BACs were anchored to  the human and mouse genome sequences by BLASTN search, revealing 29.4% and  10.1% significant hits (E < e-5), respectively. More than 60% of  all cattle BES hits in both the human and mouse genomes are located within  known genes. In order to confirm in silico predictions of orthology and their  relative position on cattle chromosomes, 84 cattle BESs with similarity to  sequences on HSA11 were mapped using a cattle–hamster radiation hybrid  (RH) panel. Resulting RH maps of BTA15 and BTA29 cover ∼85% of HSA11  sequence, revealing a complex patchwork shuffling of segments not explained by  a simple translocation followed by internal rearrangements. Overlay of the  mouse conserved syntenies onto HSA11 revealed that segmental boundaries appear  to be conserved in all three species. The BAC clone-based comparative map  provides a foundation for the evolutionary analysis of mammalian karyotypes  and for sequencing of the cattle genome. 
ER  -