@article{Yang01072003,
author = {Yang, Qinghong and Lishanski, Alla and Yang, Wendy and Hatcher, Sandra and Seet, Henrietta and Gregg, Jeffrey P.}, 
title = {Allele-Specific Holliday Junction Formation: A New Mechanism of Allelic Discrimination for SNP Scoring},
volume = {13}, 
number = {7}, 
pages = {1754-1764}, 
year = {2003}, 
doi = {10.1101/gr.997703}, 
abstract ={We report here a new mechanism for allelic
 discrimination—allele-specific Holliday Junction formation. The Holliday
 Junction (HJ) is a unique DNA structure that can be formed in a
 sequence-nonspecific manner by routine PCR. To cause the PCR-based HJ
 formation to occur in an allele-specific manner, the PCR primers are
 manipulated such that an extra mismatch next to a SNP of interest is
 introduced between a target and a reference amplicon and a GC-clamp is added.
 Based on this new mechanism, novel SNP genotyping methods were developed,
 including a homogeneous fluorescence polarization (FP) competition assay that
 requires neither labeled primers/probes nor expensive enzymes/substrates.
 Using this novel genotyping technology, we were able to convert >95% of SNP
 sequences into genotyping assays that work well under a universal set of assay
 conditions and achieved 100% accuracy in clinical samples.}, 
URL = {http://genome.cshlp.org/content/13/7/1754.abstract}, 
eprint = {http://genome.cshlp.org/content/13/7/1754.full.pdf+html}, 
journal = {Genome Research} 
}