TY  - JOUR
A1  - Thaminy, Safia
A1  - Auerbach, Daniel
A1  - Arnoldo, Anthony
A1  - Stagljar, Igor
T1  - Identification of Novel ErbB3-Interacting Factors Using the Split-Ubiquitin Membrane Yeast Two-Hybrid System
Y1  - 2003/07/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1744 
EP  - 1753 
DO  - 10.1101/gr.1276503 
VL  - 13 
IS  - 7 
UR  - http://genome.cshlp.org/content/13/7/1744.abstract 
N2  - Analysis of membrane protein interactions is difficult because of the
 hydrophobic nature of these proteins, which often renders conventional
 biochemical and genetic assays fruitless. This is a substantial problem
 because proteins that are integral or associated with membranes represent
 approximately one-third of all proteins in a typical eukaryotic cell. We have
 shown previously that the modified split-ubiquitin system can be used as a
 genetic assay for the in vivo detection of interactions between the two
 characterized yeast transmembrane proteins, Ost1p and Wbp1p. This so-called
 split-ubiquitin membrane yeast two-hybrid (YTH) system uses the
 split-ubiquitin approach in which reconstitution of two ubiquitin halves is
 mediated by a protein–protein interaction. Here we converted the
 split-ubiquitin membrane YTH system into a generally applicable in vivo
 screening approach to identify interacting partners of a particular mammalian
 transmembrane protein. We have demonstrated the effectiveness of this approach
 by using the mammalian ErbB3 receptor as bait and have identified three
 previously unknown ErbB3-interacting proteins. In addition, we have confirmed
 one of the newly found interactions between ErbB3 and the membrane-associated
 RGS4 protein by coimmunoprecipitating the two proteins from human cells. We
 expect the split-ubiquitin membrane YTH technology to be valuable for the
 identification of potential interacting partners of integral membrane proteins
 from many model organisms. 
ER  -