RT Journal
A1 Sumer, Huseyin
A1 Craig, Jeffrey M.
A1 Sibson, Mandy
A1 Choo, K.H. Andy
T1 A Rapid Method of Genomic Array Analysis of Scaffold/Matrix Attachment Regions (S/MARs) Identifies a 2.5-Mb Region of Enhanced Scaffold/Matrix Attachment at a Human Neocentromere
JF Genome Research 
JO Genome Research 
YR 2003 
FD July 01 
VO 13 
IS 7 
SP 1737 
OP 1743 
DO 10.1101/gr.1095903 
UL http://genome.cshlp.org/content/13/7/1737.abstract 
AB Human neocentromeres are fully functional centromeres that arise at
 previously noncentromeric regions of the genome. We have tested a rapid
 procedure of genomic array analysis of chromosome scaffold/matrix attachment
 regions (S/MARs), involving the isolation of S/MAR DNA and hybridization of
 this DNA to a genomic BAC/PAC array. Using this procedure, we have defined a
 2.5-Mb domain of S/MAR-enriched chromatin that fully encompasses a previously
 mapped centromere protein-A (CENP-A)-associated domain at a human
 neocentromere. We have independently verified this procedure using a
 previously established fluorescence in situ hybridization method on
 salt-treated metaphase chromosomes. In silico sequence analysis of the
 S/MAR-enriched and surrounding regions has revealed no outstanding
 sequence-related predisposition. This study defines the S/MAR-enriched domain
 of a higher eukaryotic centromere and provides a method that has broad
 application for the mapping of S/MAR attachment sites over large genomic
 regions or throughout a genome.