@article{Sumer01072003,
author = {Sumer, Huseyin and Craig, Jeffrey M. and Sibson, Mandy and Choo, K.H. Andy}, 
title = {A Rapid Method of Genomic Array Analysis of Scaffold/Matrix Attachment Regions (S/MARs) Identifies a 2.5-Mb Region of Enhanced Scaffold/Matrix Attachment at a Human Neocentromere},
volume = {13}, 
number = {7}, 
pages = {1737-1743}, 
year = {2003}, 
doi = {10.1101/gr.1095903}, 
abstract ={Human neocentromeres are fully functional centromeres that arise at
 previously noncentromeric regions of the genome. We have tested a rapid
 procedure of genomic array analysis of chromosome scaffold/matrix attachment
 regions (S/MARs), involving the isolation of S/MAR DNA and hybridization of
 this DNA to a genomic BAC/PAC array. Using this procedure, we have defined a
 2.5-Mb domain of S/MAR-enriched chromatin that fully encompasses a previously
 mapped centromere protein-A (CENP-A)-associated domain at a human
 neocentromere. We have independently verified this procedure using a
 previously established fluorescence in situ hybridization method on
 salt-treated metaphase chromosomes. In silico sequence analysis of the
 S/MAR-enriched and surrounding regions has revealed no outstanding
 sequence-related predisposition. This study defines the S/MAR-enriched domain
 of a higher eukaryotic centromere and provides a method that has broad
 application for the mapping of S/MAR attachment sites over large genomic
 regions or throughout a genome.}, 
URL = {http://genome.cshlp.org/content/13/7/1737.abstract}, 
eprint = {http://genome.cshlp.org/content/13/7/1737.full.pdf+html}, 
journal = {Genome Research} 
}