RT Journal
A1 Toyoda , Nobuaki
A1 Nagai, Shigenori
A1 Terashima, Yuya
A1 Motomura, Kazushi
A1 Haino, Makoto
A1 Hashimoto, Shin-ichi
A1 Takizawa, Hajime
A1 Matsushima, Kouji
T1 Analysis of mRNA With Microsomal Fractionation Using a SAGE-Based DNA Microarray System Facilitates Identification of the Genes Encoding Secretory Proteins
JF Genome Research 
JO Genome Research 
YR 2003 
FD July 01 
VO 13 
IS 7 
SP 1728 
OP 1736 
DO 10.1101/gr.709603 
UL http://genome.cshlp.org/content/13/7/1728.abstract 
AB In the regulation of host defense responses such as inflammation and
 immunity, the secretory proteins, including membrane proteins, play central
 roles. Although many secretory proteins have been identified by using methods
 such as differential display, random screening, or the signal sequence trap
 method, each method suffers from poor reproducibility, low sensitivity, or
 time-consuming or laborious work. Therefore, the strategy for facilitating the
 selection of the genes encoding the secretory proteins is desired. In this
 paper, we describe a system for isolating the genes encoding secretory
 proteins by analyzing mRNAs with microsomal fractionation on serial analysis
 of gene expression (SAGE)–based DNA microarray system. This system
 succeeded in discriminating the genes encoding secretory proteins from ones
 encoding nonsecretory proteins with 80% accuracy. We applied this system to
 human T lymphocytes. As a result, we were able to identify the genes that are
 not only encoding secretory proteins but also expressing selectively in a
 specific subset of T lymphocytes. The SAGE-based DNA microarray system is a
 promising system to identify the genes encoding specific secretory
 proteins.