RT Journal
A1 Ono, Ryuichi
A1 Shiura, Hirosuke
A1 Aburatani, Hiroyuki
A1 Kohda, Takashi
A1 Kaneko-Ishino, Tomoko
A1 Ishino, Fumitoshi
T1 Identification of a Large Novel Imprinted Gene Cluster on Mouse Proximal Chromosome 6
JF Genome Research 
JO Genome Research 
YR 2003 
FD July 01 
VO 13 
IS 7 
SP 1696 
OP 1705 
DO 10.1101/gr.906803 
UL http://genome.cshlp.org/content/13/7/1696.abstract 
AB Mice with maternal duplication of proximal chromosome 6 die in utero at an
 early embryonic stage. Recently, two imprinted genes, paternally expressed
 Sgce and maternally expressed Asb4, were identified in this
 region. This report analyzes the imprinting status of genes within a 1-Mb
 region containing these two genes. Peg10, which is next to
 Sgce, shows complete paternal expression, like Sgce.
 Conversely, Neurabin, Pon2, and Pon3 show preferential maternal
 expression at embryonic stages, although they all show biallelic expression in
 neonatal tissues. These results demonstrate that there is a large novel
 imprinted gene cluster in this region. 5′-RACE (Rapid Amplification of
 cDNA Ends) analysis of Peg10 revealed the existence of a novel first
 exon separate from the second exon, which encoded two putative ORFs similar to
 the viral Gag and Pol proteins. A differentially methylated region established
 in sperm and eggs is located just within the region containing the two first
 exons of Peg10 and Sgce, and may play an important role in
 regulating the two paternally expressed genes: Peg10 and
 Sgce.