TY  - JOUR
A1  - Wang, Xiaosong
A1  - Le Roy, Isabelle
A1  - Nicodeme, Edwige
A1  - Li, Renhua
A1  - Wagner, Richard
A1  - Petros, Christina
A1  - Churchill, Gary A.
A1  - Harris, Stephen
A1  - Darvasi, Ariel
A1  - Kirilovsky, Jorge
A1  - Roubertoux, Pierre L.
A1  - Paigen, Beverly
T1  - Using Advanced Intercross Lines for High-Resolution Mapping of HDL Cholesterol Quantitative Trait Loci
Y1  - 2003/07/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1654 
EP  - 1664 
DO  - 10.1101/gr.1185803 
VL  - 13 
IS  - 7 
UR  - http://genome.cshlp.org/content/13/7/1654.abstract 
N2  - Mapping quantitative trait loci (QTLs)with high resolution facilitates
 identification and positional cloning of the underlying genes. The novel
 approach of advanced intercross lines (AILs) generates many more recombination
 events and thus can potentially narrow QTLs significantly more than do
 conventional backcrosses and F2 intercrosses. In this study, we
 carried out QTL analyses in (C57BL/6J × NZB/BlNJ)× C57BL/6J
 backcross progeny fed either chow or an atherogenic diet to detect QTLs that
 regulate high-density lipoprotein cholesterol (HDL)concentrations, and in
 (C57BL/6J × NZB/BlNJ)F11 AIL progeny to confirm and narrow
 those QTLs. QTLs for HDL concentrations were found on chromosomes 1, 5, and
 16. AIL not only narrowed the QTLs significantly more than did a conventional
 backcross but also resolved a chromosome 5 QTL identified in the backcross
 into two QTLs, the peaks of both being outside the backcross QTL region. We
 tested 27 candidate genes and found significant mRNA expression differences
 for 12 (Nr1i3, Apoa2, Sap, Tgfb2, Fgfbp1, Prom, Ppargc1, Tcf1, Ncor2,
 Srb1, App, and Ifnar). Some of these underlay the same QTL,
 indicating that expression differences are common and not sufficient to
 identify QTL genes. All the major HDL QTLs in our study had homologous
 counterparts in humans, implying that their underlying genes regulate HDL in
 humans. 
ER  -