RT Journal
A1 Suzuki, Harukazu
A1 Saito, Rintaro
A1 Kanamori, Mutsumi
A1 Kai, Chikatoshi
A1 Schönbach, Christian
A1 Nagashima, Takeshi
A1 Hosaka, Junko
A1 Hayashizaki, Yoshihide
T1 The Mammalian Protein–Protein Interaction Database and Its Viewing System That Is Linked to the Main FANTOM2 Viewer
JF Genome Research 
JO Genome Research 
YR 2003 
FD June 01 
VO 13 
IS 6b 
SP 1534 
OP 1541 
DO 10.1101/gr.956303 
UL http://genome.cshlp.org/content/13/6b/1534.abstract 
AB Here, we describe the development of a mammalian protein–protein  interaction (PPI) database and of a PPI Viewer application to display protein  interaction networks  (http://fantom21.gsc.riken.go.jp/PPI/).  In the database, we stored the mammalian PPIs identified through our PPI  assays (internal PPIs), as well as those we extracted and processed (external  PPIs) from publicly available data sources, the DIP and BIND databases and  MEDLINE abstracts by using FACTS, a new functional inference and curation  system. We integrated the internal and external PPIs into the PPI database,  which is linked to the main FANTOM2 viewer. In addition, we incorporated into  the PPI Viewer information regarding the luciferase reporter activity of  internal PPIs and the data confidence of external PPIs; these data enable  visualization and evaluation of the reliability of each interaction. Using the  described system, we successfully identified several interactions of  biological significance. Therefore, the PPI Viewer is a useful tool for  exploring FANTOM2 clone-related protein interactions and their potential  effects on signaling and cellular communication.