RT Journal
A1 Nikaido, Itoshi
A1 Saito, Chika
A1 Mizuno, Yosuke
A1 Meguro, Makiko
A1 Bono, Hidemasa
A1 Kadomura, Moritoshi
A1 Kono, Tomohiro
A1 Morris, Gerard A.
A1 Lyons, Paul A.
A1 Oshimura, Mitsuo
A1 RIKEN GER Group
A1 GSL Members
A1 Hayashizaki, Yoshihide
A1 Okazaki, Yasushi
T1 Discovery of Imprinted Transcripts in the Mouse Transcriptome Using Large-Scale Expression Profiling
JF Genome Research 
JO Genome Research 
YR 2003 
FD June 01 
VO 13 
IS 6b 
SP 1402 
OP 1409 
DO 10.1101/gr.1055303 
UL http://genome.cshlp.org/content/13/6b/1402.abstract 
AB Candidate imprinted transcriptional units in the mouse genome were  identified systematically from 27,663 FANTOM2 full-length mouse cDNA clones by  expression profiling. Large-scale cDNA microarrays were used to detect  differential expression dependent upon chromosomal parent of origin by  comparing the mRNA levels in the total tissue of 9.5 dpc parthenogenote and  androgenote mouse embryos. Of the FANTOM2 transcripts, 2114 were identified as  candidates on the basis of the array data. Of these, 39 mapped to known  imprinted regions of the mouse genome, 56 were considered as nonprotein-coding  RNAs, and 159 were natural antisense transcripts. The imprinted expression of  two transcripts located in the mouse chromosomal region syntenic to the human  Prader-Willi syndrome region was confirmed experimentally. We further mapped  all candidate imprinted transcripts to the mouse and human genome and were  shown in correlation with the imprinting disease loci. These data provide a  major resource for understanding the role of imprinting in mammalian inherited  traits.