RT Journal
A1 Wells, Christine A.
A1 Ravasi, Timothy
A1 Sultana, Razvan
A1 Yagi, Ken
A1 Carninci, Piero
A1 Bono, Hidemasa
A1 Faulkner, Geoffrey
A1 Okazaki, Yasushi
A1 Quackenbush, John
A1 Hume, David A.
A1 RIKEN GER Group
A1 GSL Members
A1 Lyons, Paul A.
T1 Continued Discovery of Transcriptional Units Expressed in Cells of the Mouse Mononuclear Phagocyte Lineage
JF Genome Research 
JO Genome Research 
YR 2003 
FD June 01 
VO 13 
IS 6b 
SP 1360 
OP 1365 
DO 10.1101/gr.1056103 
UL http://genome.cshlp.org/content/13/6b/1360.abstract 
AB The current RIKEN transcript set represents a significant proportion of the  mouse transcriptome but transcripts expressed in the innate and acquired  immune systems are poorly represented. In the present study we have assessed  the complexity of the transcriptome expressed in mouse macrophages before and  after treatment with lipopolysaccharide, a global regulator of macrophage gene  expression, using existing RIKEN 19K arrays. By comparison to array profiles  of other cells and tissues, we identify a large set of macrophage-enriched  genes, many of which have obvious functions in endocytosis and phagocytosis.  In addition, a significant number of LPS-inducible genes were identified. The  data suggest that macrophages are a complex source of mRNA for transcriptome  studies. To assess complexity and identify additional macrophage expressed  genes, cDNA libraries were created from purified populations of macrophage and  dendritic cells, a functionally related cell type. Sequence analysis revealed  a high incidence of novel mRNAs within these cDNA libraries. These studies  provide insights into the depths of transcriptional complexity still untapped  amongst products of inducible genes, and identify macrophage and dendritic  cell populations as a starting point for sampling the inducible mammalian  transcriptome.