TY  - JOUR
A1  - Wells, Christine A.
A1  - Ravasi, Timothy
A1  - Sultana, Razvan
A1  - Yagi, Ken
A1  - Carninci, Piero
A1  - Bono, Hidemasa
A1  - Faulkner, Geoffrey
A1  - Okazaki, Yasushi
A1  - Quackenbush, John
A1  - Hume, David A.
A1  - RIKEN GER Group
A1  - GSL Members
A1  - Lyons, Paul A.
T1  - Continued Discovery of Transcriptional Units Expressed in Cells of the Mouse Mononuclear Phagocyte Lineage
Y1  - 2003/06/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1360 
EP  - 1365 
DO  - 10.1101/gr.1056103 
VL  - 13 
IS  - 6b 
UR  - http://genome.cshlp.org/content/13/6b/1360.abstract 
N2  - The current RIKEN transcript set represents a significant proportion of the  mouse transcriptome but transcripts expressed in the innate and acquired  immune systems are poorly represented. In the present study we have assessed  the complexity of the transcriptome expressed in mouse macrophages before and  after treatment with lipopolysaccharide, a global regulator of macrophage gene  expression, using existing RIKEN 19K arrays. By comparison to array profiles  of other cells and tissues, we identify a large set of macrophage-enriched  genes, many of which have obvious functions in endocytosis and phagocytosis.  In addition, a significant number of LPS-inducible genes were identified. The  data suggest that macrophages are a complex source of mRNA for transcriptome  studies. To assess complexity and identify additional macrophage expressed  genes, cDNA libraries were created from purified populations of macrophage and  dendritic cells, a functionally related cell type. Sequence analysis revealed  a high incidence of novel mRNAs within these cDNA libraries. These studies  provide insights into the depths of transcriptional complexity still untapped  amongst products of inducible genes, and identify macrophage and dendritic  cell populations as a starting point for sampling the inducible mammalian  transcriptome. 
ER  -