TY  - JOUR
A1  - Numata, Koji
A1  - Kanai, Akio
A1  - Saito, Rintaro
A1  - Kondo, Shinji
A1  - Adachi, Jun
A1  - Wilming, Laurens G.
A1  - Hume, David A.
A1  - RIKEN GER Group
A1  - GSL Members
A1  - Hayashizaki, Yoshihide
A1  - Tomita, Masaru
T1  - Identification of Putative Noncoding RNAs Among the RIKEN Mouse Full-Length cDNA Collection
Y1  - 2003/06/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1301 
EP  - 1306 
DO  - 10.1101/gr.1011603 
VL  - 13 
IS  - 6b 
UR  - http://genome.cshlp.org/content/13/6b/1301.abstract 
N2  - With the sequencing and annotation of genomes and transcriptomes of several  eukaryotes, the importance of noncoding RNA (ncRNA)—RNA molecules that  are not translated to protein products—has become more evident. A  subclass of ncRNA transcripts are encoded by highly regulated, multi-exon,  transcriptional units, are processed like typical protein-coding mRNAs and are  increasingly implicated in regulation of many cellular functions in  eukaryotes. This study describes the identification of candidate functional  ncRNAs from among the RIKEN mouse full-length cDNA collection, which contains  60,770 sequences, by using a systematic computational filtering approach. We  initially searched for previously reported ncRNAs and found nine murine ncRNAs  and homologs of several previously described nonmouse ncRNAs. Through our  computational approach to filter artifact-free clones that lack protein coding  potential, we extracted 4280 transcripts as the largest-candidate set. Many  clones in the set had EST hits, potential CpG islands surrounding the  transcription start sites, and homologies with the human genome. This implies  that many candidates are indeed transcribed in a regulated manner. Our results  demonstrate that ncRNAs are a major functional subclass of processed  transcripts in mammals. 
ER  -