TY  - JOUR
A1  - Carninci, Piero
A1  - Waki, Kazunori
A1  - Shiraki, Toshiyuki
A1  - Konno, Hideaki
A1  - Shibata, Kazuhiro
A1  - Itoh, Masayoshi
A1  - Aizawa, Katsunori
A1  - Arakawa, Takahiro
A1  - Ishii, Yoshiyuki
A1  - Sasaki, Daisuke
A1  - Bono, Hidemasa
A1  - Kondo, Shinji
A1  - Sugahara, Yuichi
A1  - Saito, Rintaro
A1  - Osato, Naoki
A1  - Fukuda, Shiro
A1  - Sato, Kenjiro
A1  - Watahiki, Akira
A1  - Hirozane-Kishikawa, Tomoko
A1  - Nakamura, Mari
A1  - Shibata, Yuko
A1  - Yasunishi, Ayako
A1  - Kikuchi, Noriko
A1  - Yoshiki, Atsushi
A1  - Kusakabe, Moriaki
A1  - Gustincich, Stefano
A1  - Beisel, Kirk
A1  - Pavan, William
A1  - Aidinis, Vassilis
A1  - Nakagawara, Akira
A1  - Held, William A.
A1  - Iwata, Hiroo
A1  - Kono, Tomohiro
A1  - Nakauchi, Hiromitsu
A1  - Lyons, Paul
A1  - Wells, Christine
A1  - Hume, David A.
A1  - Fagiolini, Michela
A1  - Hensch, Takao K.
A1  - Brinkmeier, Michelle
A1  - Camper, Sally
A1  - Hirota, Junji
A1  - Mombaerts, Peter
A1  - Muramatsu, Masami
A1  - Okazaki, Yasushi
A1  - Kawai, Jun
A1  - Hayashizaki, Yoshihide
T1  - Targeting a Complex Transcriptome: The Construction of the Mouse Full-Length cDNA Encyclopedia
Y1  - 2003/06/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 1273 
EP  - 1289 
DO  - 10.1101/gr.1119703 
VL  - 13 
IS  - 6b 
UR  - http://genome.cshlp.org/content/13/6b/1273.abstract 
N2  - We report the construction of the mouse full-length cDNA encyclopedia,the  most extensive view of a complex transcriptome,on the basis of preparing and  sequencing 246 libraries. Before cloning,cDNAs were enriched in full-length by  Cap-Trapper,and in most cases,aggressively subtracted/normalized. We have  produced 1,442,236 successful 3′-end sequences clustered into 171,144  groups, from which 60,770 clones were fully sequenced cDNAs annotated in the  FANTOM-2 annotation. We have also produced 547,149 5′ end reads,which  clustered into 124,258 groups. Altogether, these cDNAs were further grouped in  70,000 transcriptional units (TU),which represent the best coverage of a  transcriptome so far. By monitoring the extent of normalization/subtraction,  we define the tentative equivalent coverage (TEC),which was estimated to be  equivalent to >12,000,000 ESTs derived from standard libraries. High  coverage explains discrepancies between the very large numbers of clusters  (and TUs) of this project,which also include non-protein-coding RNAs,and the  lower gene number estimation of genome annotations. Altogether,5′-end  clusters identify regions that are potential promoters for 8637 known genes  and 5′-end clusters suggest the presence of almost 63,000  transcriptional starting points. An estimate of the frequency of  polyadenylation signals suggests that at least half of the singletons in the  EST set represent real mRNAs. Clones accounting for about half of the  predicted TUs await further sequencing. The continued high-discovery rate  suggests that the task of transcriptome discovery is not yet complete. 
ER  -