RT Journal
A1 Cahill, Patrick
A1 Bakis, Michele
A1 Hurley, James
A1 Kamath, Veena
A1 Nielsen, William
A1 Weymouth, Dina
A1 Dupuis, Josée
A1 Doucette-Stamm, Lynn
A1 Smith, Douglas R.
T1 Exo-Proofreading, A Versatile SNP Scoring Technology
JF Genome Research 
JO Genome Research 
YR 2003 
FD May 01 
VO 13 
IS 5 
SP 925 
OP 931 
DO 10.1101/gr.939903 
UL http://genome.cshlp.org/content/13/5/925.abstract 
AB We report the validation of a new assay for typing single nucleotide polymorphisms (SNPs) that takes advantage of the 3′-to-5′ exonuclease proofreading activity of many DNA polymerases. The assay uses one or more primers labeled on the 3′ nucleotide base, and can be implemented in a variety of formats including a one-step PCR reaction that allows SNP typing directly from genomic DNA samples. The detection of genotypes can be accomplished by means of fluorescence detection on assays that have been purified to remove excess primer, or by means of fluorescence polarization without any additional cleanup. We also demonstrate that the Exo-Proofreading SNP assay can be used on pooled samples to obtain allele frequency data.