TY  - JOUR
A1  - Till, Bradley J.
A1  - Reynolds, Steven H.
A1  - Greene, Elizabeth A.
A1  - Codomo, Christine A.
A1  - Enns, Linda C.
A1  - Johnson, Jessica E.
A1  - Burtner, Chris
A1  - Odden, Anthony R.
A1  - Young, Kim
A1  - Taylor, Nicholas E.
A1  - Henikoff, Jorja G.
A1  - Comai, Luca
A1  - Henikoff, Steven
T1  - Large-Scale Discovery of Induced Point Mutations With High-Throughput TILLING
Y1  - 2003/03/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 524 
EP  - 530 
DO  - 10.1101/gr.977903 
VL  - 13 
IS  - 3 
UR  - http://genome.cshlp.org/content/13/3/524.abstract 
N2  - TILLING (Targeting Induced LocalLesions in Genomes) is a general reverse-genetic strategy that provides an allelic series of induced point mutations in genes of interest. High-throughput TILLING allows the rapid and low-cost discovery of induced point mutations in populations of chemically mutagenized individuals. As chemical mutagenesis is widely applicable and mutation detection for TILLING is dependent only on sufficient yield of PCR products, TILLING can be applied to most organisms. We have developed TILLING as a service to the Arabidopsis community known as the ArabidopsisTILLING Project (ATP). Our goal is to rapidly deliver allelic series of ethylmethanesulfonate-induced mutations in target 1-kb loci requested by the international research community. In the first year of public operation, ATP has discovered, sequenced, and delivered >1000 mutations in >100 genes ordered by Arabidopsis researchers. The tools and methodologies described here can be adapted to create similar facilities for other organisms. 
ER  -