RT Journal
A1 Gotoh, Koshichi
A1 Oishi, Michio
T1 Screening of Gene-Associated Polymorphisms by Use of In-Gel Competitive Reassociation and EST (cDNA) Array Hybridization
JF Genome Research 
JO Genome Research 
YR 2003 
FD March 01 
VO 13 
IS 3 
SP 492 
OP 495 
DO 10.1101/gr.434103 
UL http://genome.cshlp.org/content/13/3/492.abstract 
AB In-gel competitive reassociation (IGCR) is a method of differential subtraction to enrich polymorphic DNA restriction fragments between two DNA samples without probes or specific sequence information. Here, we show that by combining IGCR and expressed sequence tags (EST) array hybridization, polymorphic DNA fragments associated with genes in complex higher organisms (Arabidopsis thaliana) can be effectively screened, demonstrating that this procedure offers a simple and efficient way to obtain gene-associated polymorphic DNA markers.[The following individuals kindly provided reagents, samples, or unpublished information as indicated in the paper: N. Sakurai, D. Shibata, and C. Kuwata.]