RT Journal
A1 Förster, Jochen
A1 Famili, Iman
A1 Fu, Patrick
A1 Palsson, Bernhard Ø.
A1 Nielsen, Jens
T1 Genome-Scale Reconstruction of the Saccharomyces cerevisiae Metabolic Network
JF Genome Research 
JO Genome Research 
YR 2003 
FD February 01 
VO 13 
IS 2 
SP 244 
OP 253 
DO 10.1101/gr.234503 
UL http://genome.cshlp.org/content/13/2/244.abstract 
AB The metabolic network in the yeast Saccharomyces cerevisiaewas reconstructed using currently available genomic, biochemical, and physiological information. The metabolic reactions were compartmentalized between the cytosol and the mitochondria, and transport steps between the compartments and the environment were included. A total of 708 structural open reading frames (ORFs) were accounted for in the reconstructed network, corresponding to 1035 metabolic reactions. Further, 140 reactions were included on the basis of biochemical evidence resulting in a genome-scale reconstructed metabolic network containing 1175 metabolic reactions and 584 metabolites. The number of gene functions included in the reconstructed network corresponds to ∼16% of all characterized ORFs in S. cerevisiae. Using the reconstructed network, the metabolic capabilities of S. cerevisiae were calculated and compared with Escherichia coli. The reconstructed metabolic network is the first comprehensive network for a eukaryotic organism, and it may be used as the basis for in silico analysis of phenotypic functions.[Supplemental material is available online at www.genome.org. The detailed genome-scale reconstructed model ofSaccharomyces cerevisiae can be found athttp://www.cpb.dtu.dk/models/yeastmodel.html orhttp://geneticcircuits.ucsd.edu/organisms/yeast.html.]