RT Journal
A1 Ren, Chengwei
A1 Lee, Mi-Kyung
A1 Yan, Bo
A1 Ding, Kejiao
A1 Cox, Bettye
A1 Romanov, Michael N.
A1 Price, Jennifer A.
A1 Dodgson, Jerry B.
A1 Zhang, Hong-Bin
T1 A BAC-Based Physical Map of the Chicken Genome
JF Genome Research 
JO Genome Research 
YR 2003 
FD December 01 
VO 13 
IS 12 
SP 2754 
OP 2758 
DO 10.1101/gr.1499303 
UL http://genome.cshlp.org/content/13/12/2754.abstract 
AB A genome-wide physical map constructed with bacterial artificial chromosomes (BACs) is an essential component in linking phenotypic traits to the responsible genetic variation in the genomes of plants and animals. We have constructed a physical map of the chicken genome from 57,091 BACs (7.9-fold haploid genome coverage) by restriction fingerprint analysis using high-resolution polyacrylamide gel electrophoresis. The physical map consists of 2331 overlapping BAC contigs and is estimated to span 1510 Mb in physical length. BAC contigs were verified manually and by screening the BACs with 367 DNA markers. A total of 361 of the contigs have been anchored to the existing chicken genetic map. This map represents the first genome-wide, BAC-based physical map of the chicken genome. It provides a powerful platform for many areas of chicken genomics, including targeted marker development, fine mapping of genes and QTL alleles, positional cloning, analysis of avian genome organization and evolution, chicken-mammalian comparative genomics, and large-scale genome sequencing.