RT Journal
A1 Vettore, André L.
A1 da Silva, Felipe R.
A1 Kemper, Edson L.
A1 Souza, Glaucia M.
A1 da Silva, Aline M.
A1 Ferro, Maria Inês T.
A1 Henrique-Silva, Flavio
A1 Giglioti, Éder A.
A1 Lemos, Manoel V.F.
A1 Coutinho, Luiz L.
A1 Nobrega, Marina P.
A1 Carrer, Helaine
A1 França, Suzelei C.
A1 Bacci, Maurício
A1 Goldman, Maria Helena S.
A1 Gomes, Suely L.
A1 Nunes, Luiz R.
A1 Camargo, Luis E.A.
A1 Siqueira, Walter J.
A1 Van Sluys, Marie-Anne
A1 Thiemann, Otavio H.
A1 Kuramae, Eiko E.
A1 Santelli, Roberto V.
A1 Marino, Celso L.
A1 Targon, Maria L.P.N.
A1 Ferro, Jesus A.
A1 Silveira, Henrique C.S.
A1 Marini, Danyelle C.
A1 Lemos, Eliana G.M.
A1 Monteiro-Vitorello, Claudia B.
A1 Tambor, José H.M.
A1 Carraro, Dirce M.
A1 Roberto, Patrícia G.
A1 Martins, Vanderlei G.
A1 Goldman, Gustavo H.
A1 de Oliveira, Regina C.
A1 Truffi, Daniela
A1 Colombo, Carlos A.
A1 Rossi, Magdalena
A1 de Araujo, Paula G.
A1 Sculaccio, Susana A.
A1 Angella, Aline
A1 Lima, Marleide M.A.
A1 de Rosa, Vicente E.
A1 Siviero, Fábio
A1 Coscrato, Virginia E.
A1 Machado, Marcos A.
A1 Grivet, Laurent
A1 Di Mauro, Sonia M.Z.
A1 Nobrega, Francisco G.
A1 Menck, Carlos F.M.
A1 Braga, Marilia D.V.
A1 Telles, Guilherme P.
A1 Cara, Frank A.A.
A1 Pedrosa, Guilherme
A1 Meidanis, João
A1 Arruda, Paulo
T1 Analysis and Functional Annotation of an Expressed Sequence Tag Collection for Tropical Crop Sugarcane
JF Genome Research 
JO Genome Research 
YR 2003 
FD December 01 
VO 13 
IS 12 
SP 2725 
OP 2735 
DO 10.1101/gr.1532103 
UL http://genome.cshlp.org/content/13/12/2725.abstract 
AB To contribute to our understanding of the genome complexity of sugarcane, we undertook a large-scale expressed sequence tag (EST) program. More than 260,000 cDNA clones were partially sequenced from 26 standard cDNA libraries generated from different sugarcane tissues. After the processing of the sequences, 237,954 high-quality ESTs were identified. These ESTs were assembled into 43,141 putative transcripts. Of the assembled sequences, 35.6% presented no matches with existing sequences in public databases. A global analysis of the whole SUCEST data set indicated that 14,409 assembled sequences (33% of the total) contained at least one cDNA clone with a full-length insert. Annotation of the 43,141 assembled sequences associated almost 50% of the putative identified sugarcane genes with protein metabolism, cellular communication/signal transduction, bioenergetics, and stress responses. Inspection of the translated assembled sequences for conserved protein domains revealed 40,821 amino acid sequences with 1415 Pfam domains. Reassembling the consensus sequences of the 43,141 transcripts revealed a 22% redundancy in the first assembling. This indicated that possibly 33,620 unique genes had been identified and indicated that >90% of the sugarcane expressed genes were tagged.