TY  - JOUR
A1  - Lee, Dong-ki
A1  - Park, Jin Woo
A1  - Kim, Youn-Jae
A1  - Kim, Jiwon
A1  - Lee, Yangsoon
A1  - Kim, Jeonglim
A1  - Kim, Jin-Soo
T1  - Toward a Functional Annotation of the Human Genome Using Artificial Transcription Factors
Y1  - 2003/12/01 
JF  - Genome Research 
JO  - Genome Research 
SP  - 2708 
EP  - 2716 
DO  - 10.1101/gr.1397903 
VL  - 13 
IS  - 12 
UR  - http://genome.cshlp.org/content/13/12/2708.abstract 
N2  - We have developed a novel, high-throughput approach to collecting randomly perturbed gene-expression profiles from the human genome. A human 293 cell library that stably expresses randomly chosen zinc-finger transcription factors was constructed, and the expression profile of each cell line was obtained using cDNA microarray technology. Gene expression profiles from a total of 132 cell lines were collected and analyzed by (1) a simple clustering method based on expression-profile similarity, and (2) the shortest-path analysis method. These analyses identified a number of gene groups, and further investigation revealed that the genes that were grouped together had close biological relationships. The artificial transcription factor-based random genome perturbation method thus provides a novel functional genomic tool for annotation and classification of genes in the human genome and those of many other organisms. 
ER  -