@article{Lee01122003,
author = {Lee, Dong-ki and Park, Jin Woo and Kim, Youn-Jae and Kim, Jiwon and Lee, Yangsoon and Kim, Jeonglim and Kim, Jin-Soo}, 
title = {Toward a Functional Annotation of the Human Genome Using Artificial Transcription Factors},
volume = {13}, 
number = {12}, 
pages = {2708-2716}, 
year = {2003}, 
doi = {10.1101/gr.1397903}, 
abstract ={We have developed a novel, high-throughput approach to collecting randomly perturbed gene-expression profiles from the human genome. A human 293 cell library that stably expresses randomly chosen zinc-finger transcription factors was constructed, and the expression profile of each cell line was obtained using cDNA microarray technology. Gene expression profiles from a total of 132 cell lines were collected and analyzed by (1) a simple clustering method based on expression-profile similarity, and (2) the shortest-path analysis method. These analyses identified a number of gene groups, and further investigation revealed that the genes that were grouped together had close biological relationships. The artificial transcription factor-based random genome perturbation method thus provides a novel functional genomic tool for annotation and classification of genes in the human genome and those of many other organisms.}, 
URL = {http://genome.cshlp.org/content/13/12/2708.abstract}, 
eprint = {http://genome.cshlp.org/content/13/12/2708.full.pdf+html}, 
journal = {Genome Research} 
}