RT Journal
A1 Sousa-Nunes, Rita
A1 Rana, Amer Ahmed
A1 Kettleborough, Ross
A1 Brickman, Joshua M.
A1 Clements, Melanie
A1 Forrest, Alistair
A1 Grimmond, Sean
A1 Avner, Philip
A1 Smith, James C.
A1 Dunwoodie, Sally L.
A1 Beddington, Rosa S.P.
T1 Characterizing Embryonic Gene Expression Patterns in the Mouse Using Nonredundant Sequence-Based Selection
JF Genome Research 
JO Genome Research 
YR 2003 
FD December 01 
VO 13 
IS 12 
SP 2609 
OP 2620 
DO 10.1101/gr.1362303 
UL http://genome.cshlp.org/content/13/12/2609.abstract 
AB This article investigates the expression patterns of 160 genes that are expressed during early mouse development. The cDNAs were isolated from 7.5 d postcoitum (dpc) endoderm, a region that comprises visceral endoderm (VE), definitive endoderm, and the node–tissues that are required for the initial steps of axial specification and tissue patterning in the mouse. To avoid examining the same gene more than once, and to exclude potentially ubiquitously expressed housekeeping genes, cDNA sequence was derived from 1978 clones of the Endoderm library. These yielded 1440 distinct cDNAs, of which 123 proved to be novel in the mouse. In situ hybridization analysis was carried out on 160 of the cDNAs, and of these, 29 (18%) proved to have restricted expression patterns.