RT Journal
A1 Zavolan, Mihaela
A1 van Nimwegen, Erik
A1 Gaasterland, Terry
T1 Splice Variation in Mouse Full-Length cDNAs Identified by Mapping to the Mouse Genome
JF Genome Research 
JO Genome Research 
YR 2002 
FD September 01 
VO 12 
IS 9 
SP 1377 
OP 1385 
DO 10.1101/gr.191702 
UL http://genome.cshlp.org/content/12/9/1377.abstract 
AB We mapped the collection of The Institute of Physical and Chemical Research (Japan) (RIKEN) 21,076 full-length mouse cDNA clone sequences and the mouse RefSeq sequences to the recently completed draft of the mouse genome. Using this mapping, we identified 3674 mouse genes with multiple transcripts, of which 1098 have splice variants. All but 532 of 21,076 clones (97.5%) mapped to the genome assembly. Alignments of cDNA clone sequences with proteins show that much of the detected splice variation alters coding regions and affects the translated protein. We developed novel analytical techniques to classify observed splice variation and to assess the relation between splice variation and alternative transcription. This analysis indicates that an alternative choice of transcription start or polyadenylation signal frequently induces splice variation.