RT Journal
A1 Stapleton, Mark
A1 Liao, Guochun
A1 Brokstein, Peter
A1 Hong, Ling
A1 Carninci, Piero
A1 Shiraki, Toshiyuki
A1 Hayashizaki, Yoshihide
A1 Champe, Mark
A1 Pacleb, Joanne
A1 Wan, Ken
A1 Yu, Charles
A1 Carlson, Joe
A1 George, Reed
A1 Celniker, Susan
A1 Rubin, Gerald M.
T1 The Drosophila Gene Collection: Identification of Putative Full-Length cDNAs for 70% of D. melanogaster Genes
JF Genome Research 
JO Genome Research 
YR 2002 
FD August 01 
VO 12 
IS 8 
SP 1294 
OP 1300 
DO 10.1101/gr.269102 
UL http://genome.cshlp.org/content/12/8/1294.abstract 
AB Collections of full-length nonredundant cDNA clones are critical reagents for functional genomics. The first step toward these resources is the generation and single-pass sequencing of cDNA libraries that contain a high proportion of full-length clones. The first release of the Drosophila Gene Collection Release 1 (DGCr1) was produced from six libraries representing various tissues, developmental stages, and the cultured S2 cell line. Nearly 80,000 random 5′ expressed sequence tags (5′ expressed sequence tags [ESTs]from these libraries were collapsed into a nonredundant set of 5849 cDNAs, corresponding to ∼40% of the 13,474 predicted genes in Drosophila. To obtain cDNA clones representing the remaining genes, we have generated an additional 157,835 5′ ESTs from two previously existing and three new libraries. One new library is derived from adult testis, a tissue we previously did not exploit for gene discovery; two new cap-trapped normalized libraries are derived from 0–22-h embryos and adult heads. Taking advantage of the annotated D. melanogaster genome sequence, we clustered the ESTs by aligning them to the genome. Clusters that overlap genes not already represented by cDNA clones in the DGCr1 were analyzed further, and putative full-length clones were selected for inclusion in the new DGC. This second release of the DGC (DGCr2) contains 5061 additional clones, extending the collection to 10,910 cDNAs representing >70% of the predicted genes inDrosophila.[The sequence data described in this paper have been submitted to the GenBank data library under accession nos. BF485518-BF503517, BF503521-BF506780, BG631888-BG631996,BG633696-BG637540, BG640063-BG641469, BI141709-BI142246,BI161485-BI173971, BI212109-BI216987, BI227448-BI233322,BI234009-BI243989, BI351612-BI354228, BI354231-BI355901,BI355935-BI358751, BI361285-BI376197, BI481532-BI487261,BI563331-BI593695, BI604243-BI620155, BI620158-BI635012,BI635064-BI638027, and BI638030-BI642053. The following individuals kindly provided reagents, samples, or unpublished information as indicated in the paper: J. Pringle and M. Fuller.]