RT Journal
A1 Kochiwa, Hiromi
A1 Suzuki, Ryosuke
A1 Washio, Takanori
A1 Saito, Rintaro
A1 Phase, The RIKEN Genome Exploration Research Group
A1 Bono, Hidemasa
A1 Carninci, Piero
A1 Okazaki, Yasushi
A1 Miki, Rika
A1 Hayashizaki, Yoshihide
A1 Tomita, Masaru
T1 Inferring Alternative Splicing Patterns in Mouse from a Full-Length cDNA Library and Microarray Data
JF Genome Research 
JO Genome Research 
YR 2002 
FD August 01 
VO 12 
IS 8 
SP 1286 
OP 1293 
DO 10.1101/gr.220302 
UL http://genome.cshlp.org/content/12/8/1286.abstract 
AB Although many studies on alternative splicing of specific genes have been reported in the literature, the general mechanism that regulates alternative splicing has not been clearly understood. In this study, we systematically aligned each pair of the 21,076 cDNA sequences ofMus musculus, searched for putative alternative splicing patterns, and constructed a list of potential alternative splicing sites. Two cDNAs are suspected to be alternatively spliced and originating from a common gene if they share most of their region with a high degree of sequence homology, but parts of the sequences are very distinctive or deleted in either cDNA. The list contains the following information: (1) tissue, (2) developmental stage, (3) sequences around splice sites, (4) the length of each gapped region, and (5) other comments. The list is available athttp://www.bioinfo.sfc.keio.ac.jp/intron. Our results have predicted a number of unreported alternatively spliced genes, some of which are expressed only in a specific tissue or at a specific developmental stage.